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 Bio-safety Level 3 Laboratory

BSL-3 is suitable for work with infectious agents which may cause serious or potentially lethal diseases as a result of exposure by the inhalation route. BSL-3 laboratories should be located away from high-traffic areas. Examples of agents that should be manipulated at BSL-3 are M. tuberculosis (research activities), St. Louis encephalitis virus, and Coxiella burnetii).

These laboratories are characterized by having a double-door entry. Because the agents manipulated at BSL-3 are transmissible by the aerosol route, particular attention is given to air movement in these labs. Air moves from areas of lesser contamination to areas of higher contamination, such as from the corridor into the laboratory. Air movement is also single pass; exhaust air is not re-circulated to other rooms. Exhaust air does not have to be HEPA filtered, unless local conditions are such that re-entrainment into building air supply systems is unavoidable.

All work that may create aerosols or splatter is done inside a biological safety cabinet. Wall, ceiling and floor penetrations are sealed to keep aerosols in and to keep gaseous decontaminants in. The floor is monolithic, and there are continuous cove moldings that extend at least 4 up the wall. Acoustic tiles are not used in BSL-3 laboratories; ceilings should be waterproof for ease of cleaning Centrifuge tubes are placed into containment cups or heads in the BSC, transferred to the centrifuge, spun, and then returned to the BSC to be unloaded. In some laboratories the centrifuges themselves are enclosed in a vented area to minimize possible aerosol exposures created in the event of a centrifuge failure. Vacuum lines are protected with HEPA filters so that maintenance personnel are not exposed to infectious aerosols.

Class II type-A biological safety cabinets are suitable in BSL-3 laboratories. Sometimes Class II type B3 cabinets are installed, requiring thimble connection to the building exhaust systems. Depending on the nature of the work being done in the BSL-3 laboratory, additional personnel protective devices may be worn, such as respirators. When pulmonary protection is required, the laboratorians need to have appropriate medical evaluations and be trained in proper fit testing and care of their respirators.

Supervisors of BSL-3 laboratories should be competent scientists experienced in working with the agents. They establish criteria for entry into the laboratory, restrict access, develop appropriate practices and procedures, and train the lab technicians.  They are also responsible for developing the laboratory safety manual. The lab personnel must rigorously follow the established guidelines, demonstrate proficiency in performing their various procedures, and receive appropriate training. They must participate in specified medical surveillance programs, and report all incidents that constitute potential exposures.


Programme of Work
Molecular Genetics
Animal Nutrition
Quality Control
Bio-safety Level 3 Lab
Animal Bio safety Level 3 Lab
C I Laboratory
Bio-informatics & IT Centre
Library Information Centre
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Livestock Complex
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